POWERED BY CAMAG HPTLC SOFTWARE
high-precision quantitative evaluation
- Advanced Software Integration: The visionCATS HPTLC software seamlessly integrates with the TLC Scanner 4, providing an intuitive platform for data acquisition, analysis, and reporting. It enables the automatic execution of multiple scanning and evaluation steps.
- Versatile Detection Capabilities: The TLC Scanner 4 can operate in both UV and visible light spectrums, covering a broad wavelength range from 190 to 900 nm. This versatility allows the detection of various types of substances, expanding the scope of analysis to more complex samples.
- Multi-Wavelength and Multi-Step Evaluation: The combination of the TLC Scanner 4 and visionCATS allows for multi-wavelength scanning and multi-step evaluations. Users can analyze a plate under different conditions, such as before and after derivatization, or at multiple wavelengths, enhancing the ability to differentiate and quantify closely related or co-eluting substances.
- Data Integrity and Regulatory Compliance: visionCATS is designed with compliance in mind, meeting the standards required in regulated environments, including GLP and GMP settings. Its built-in security features ensure data integrity, audit trails, and traceability, which are essential for laboratory environments that need to comply with industry regulations.
easy and intuitive operation
01
Definition: In the first step, the substances to be analyzed and their concentration in the reference vials are defined. The quantity applied of each reference will be calculated from the application volume and the defined concentration.
02
Integration: If required, the peak detection is configured, based on profiles issued from the execution of a scanner step.
03
Substance assignment: When the substances and the peaks are defined, they have to be associated. For a substance assigned to a peak belonging to a reference application, the height or the area of the peak is associated with the quantity applied.
04
Calibration: For a given substance and for the reference tracks, the set of couples (quantity applied, height or area of the peak) are projected on an XY plan, and a regression algorithm is used to find an appropriate regression curve for these reference points. Peaks coming from sample tracks also have a height (and an area). The regression algorithm calculates the quantity of the substance applied in the sample.
05
Results: A summary of the quantified amounts/concentrations of each substance in the sample(s) is displayed. The quantity of substance applied in each sample application computed during the calibration, associated with the applied volume, gives the estimated concentration of each searched substance in each sample vial concerned.
Hardware insights
THE OPTICAL SYSTEM
- Any of the three light sources, high pressure mercury lamp, deuterium lamp, or halogen-tungsten lamp can be positioned in the light path by a motor drive. (1)
- The signal of the measuring photomultiplier is continuously offset against the signal of the reference photomultiplier. (4 and 5) This compensates for lamp aging and short-time fluctuations. It also reduces the warm-up time required to reach lamp stabilization.
- All components of the optical system, lamps, monochromator, scanning stage, and photomultiplier are mounted on one sturdy metal support. This ensures high precision of the detector signal.
- For scanning at wavelengths below 200 nm it is advisable to flush the monochromator with nitrogen. The TLC Scanner 4 is equipped to do this.
- A monochromator bandwidth of 5 nm or 20 nm can be selected. 5 nm bandwidth is used for spectra recording, multi-wavelength scanning, and when spectral selectivity is required. (2) 20 nm bandwidth offers higher light intensity (improves the signal to noise ratio and thus the reproducibility of the measurement) and enables measurement of several fractions with slightly different absorption maxima in one scan.
- The lens system with 190 – 900 nm transmission range features automatic positioning for micro and macro slit sizes. This ensures that the light energy available with small slits in the micro position is almost the same as that for the corresponding slit in the macro position, which is four times larger. (3)
- The light beam strikes the object at right angle. The photomultiplier is aligned at an angle of 30°. (5)
