Cumulative CAMAG
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J. Food Sci. 38, 185-201 (2025). HPTLC of monoacylglycerol, diacylglycerol and triacylglycerol in Brazilian refined peanut oil on silica gel with hexane-diethyl ether-formic acid 70:30:1. Detection under UV light at 254 and 366 nm. The zones were scrapped from the plate and further analyzed by gas chromatography.

Marine Drugs 23(9), 352 (2025). Summary: This review discusses the TLC-based rapid detection techniques developed for specific subclasses of marine glycolipids in complex biological samples, as well as their place compared to other analytical methods that can be hyphenated to MS, such as liquid chromatography (LC), hydrophilic interaction liquid chromatography (HILIC), reverse-phase liquid chromatography (RPLC) or supercritical fluid chromatography (SFC). Qualitative and quantitative TLC analysis was discussed for: (A) Separation of subclasses of diacylglycerols, of gangliosides, of glycosylceramides, by choosing and modifying the mobile phases; or by using two-dimension TLC, or automated multiple development (AMD). (B) Optimization of the stationary phase: e.g. by impregnation with boric acid. (C) Visualization with carbohydrate-specific reagents: primuline reagent; iodine vapour; orcinol – sulphuric acid for neutral sugar moieties; immunostaining or resorcinol hydrochloric acid for gangliosides; Azure A (cationic methylated thiazine) for sulfatides or sulfated glycolipids. (D) Quantification by fluorescence densitometry, by immunostaining, or by flame ionization detection: this last method is usually applied after AMD, because successive mobile phases will optimize the separation of glycolipids of different polarities. (E) Hyphenation to MS through soft ionisation methods, such as matrix-assisted laser desorption ionisation (MALDI) under vacuum, or elution-based electrospray ionisation MS interfaces at ambient pressures (including Desorption electrospray ionisation DESI and Liquid extraction surface analysis LESA). Methods are compared in terms of formation of complex adducts, of interferences of matrix molecules (decreased when using grapheme matrices), of undue fragmenta.

Marine Drugs 23(6), 224 (2025). Summary: Samples were Soxhlet extracts of seaweed Dictyota bartayresiana (Dictyotaceae). TLC and HPTLC on silica gel with ethyl acetate – formic acid – acetic acid – water  (100:11:11:26) (MP1), and with chloroform – methanol – formic acid (17:2:1) (MP2), showed red bands corresponding to flavonoids by visualization under UV light at 254nm, and one dark band (hRF 82-87 with MP2) at UV 366nm on fluorescent layer. Standards were flavonoids, among which myricetin and galangin (hRF 13 and 61, respectively, with MP2).

Marine Drugs 22(12), 569 (2024). Summary: A new alginate lyase, isolated from marine bacterium Microbulbifer sp. strain HZ11 (Alteromonadaceae), was incubated (from 0h to 60h at 30°C, in 50 mM sodium phosphate and 0.3 M sodium chloride, pH 7.5) with sodium alginate, or with related mannuronate and guluronate polymers (polyM and polyG). Reaction products, as well as saccharides with different polymerisation degrees (PD 1 – 5) as standards, were developed on TLC silica gel with n-butanol ­– acetic acid – water (3:2:3). Derivatization by spraying sulfuric acid (10% in ethanol), followed by 5min heating at 110°C. Visualization under white light. Oligosaccharides could be detected after 2 h of enzymatic hydrolysis: mainly PD 1 – 2, and saturation at 24h and 48h, respectively, for alginate and polyG; mainly PD 2 – 3, and saturation at 36h, for polyM.

Marine Drugs 22(12), 558 (2024). Summary: Recombinant Spg103, an enzyme isolated from marine bacterium Streptomyces sp. strain J103 (Streptomycetaceae), was incubated (from 0h to 4h at 60°C) with β-glucan, or with lichenan, or with carboxymethyl-cellulose (CMC), or with standards dextrose, cellobiose, cellotriose, cellopentose, cellohexose. Reaction products, as well as standards, were developed on TLC silica gel with n-butanol ­– ethanol – water (3:2:2). Derivatization by spraying orcinol reagent (0.05% in acetone, with 5% sulfuric acid), followed by 10min heating at 110°C. Visualization under white light. Oligosaccharides could be detected already after 1h of enzymatic hydrolysis: with β-glucan and with lichenan, the main product after 2h was the trimer. However, with CMC, the activity was very low, the main products being the hexamer and dimer, no bands for trimer, tetramer or pentamer were observed. Standards were also degraded by the enzyme: the hexamer, into dimer; the pentamer, into trimer and dimer; the trimer, into dimer and dextrose. These results indicated a double activity in the enzyme: (1) a rapid endo-activity for β-glucan or with lichenan (both containing both β-1,2- and β-1,4-bonds) or with oligomers; (2) a slower exo-activity exclusively for the β-1,4-bond of hexamers and higher polymers like CMC. This was a distinctive property not observed in typical lichenases.