Cumulative CAMAG
Bibliography Service CCBS

PLoS One 20(5), e0324390 (2025).
Samples were polar lipids extracted from Cupriavidus consociatus and C. oxalaticus (Burkholderiaceae) through the Bligh and Dyer’s technique. Two-dimensional TLC on silica gel with chloroform – methanol – water 14:6:1, followed by chloroform – methanol – acetic acid 13:5:2. Derivatization by spraying with ninhydrine for amino-lipids (phosphatidylethanolamine was detected in all strains), or with periodate Schiff’s reagent for glycolipids (phosphatidylglycerol was detected in C. consociatus, and cardiolipin was detected in both species).

Food Chem. 493, 145746 (2025). HPTLC of lipids (free fatty acids, acylglycerols, sterols, sterol esters, glycolipids, phospholipids, tocopherol), diterpenes (cafestol and kahweol), and alkaloids (caffeine and theobromine) in coffee leaves on silica gel with chloroform - methanol - water 34:8:1 for polar compounds; chloroform - methanol - water 180:20:3 for alkaloids; and hexane - diethyl ether - acetic acid 75:25:1 for non-polar compounds. Detection by spraying with cupper sulfate - phosphoric acid - methanol - water 10:8:5:78 or thymol or ninhydrin reagent. Qualitative identification under UV light at 254 and 366 nm. 

PLoS One 20(5), e0324608 (2025).
TLC on silica gel 1) to monitor the synthesis of dinitrobenzoic acid derivatives, and 2) to investigate their antimycobacterial mechanism. For 1) method A) with dichloromethane – methanol 93:7 for methyl 3,5-dinitrobenzoate (hRF 70), its hydrazide (hRF 30), and its semicarbazides = hydrazine-carboxamides (hRF 40-56) and thiosemicarbazide = hydrazine-carbothioamide (hRF 36); B) with dichloromethane – methanol 97:3 for oxadiazolamide derivatives (hRF 29-70) and for thiadiazolamide derivative (hRF 84); C) with pure chloroform or with n-hexane – ethyl acetate 1:1 for oxadiazolamines and thiadiazolamines (hRF 17-79). Detection under UV 254 nm.  For 2) lipids were isolated from Mycobacterium tuberculosis (Mycobacteriaceae) after 24h culture with radioactive 14C-acetate, in a growth medium untreated or treated (four of the synthetized derivatives were tested). TLC separation with chloroform – methanol – water 40:8:1. Visualization through autoradiography in an automated imager. In treated cells, trehalose monomycolates and dimycolates were accumulated, whereas phosphatidylethanolamine (PEA) was slightly increased and cardiolipin was unchanged. Therefore, the proposed mechanism was an inhibition of epimerase DprE1.

J. Planar Chromatogr. 37, 261-269 (2024). HPTLC of amlodipine besylate (1) and azilsartan (2) mixture in human plasma on silica gel with toluene - ethyl acetate - methanol - acetone - acetic acid 12:3:2:1:2. Quantitative determination by absorbance measurement at 244 nm. The hRF values for (1) and (2) were 22 and 63, respectively. Linearity was between 60 and 600 ng/zone for (1) and 90 and 900 ng/zone for (2). The inter-day and intra-day precision was below 3 % (n=6). The LOD and LOQ were 14 and 42 ng/zone for (1) and 19 and 56 ng/zone for (2), respectively. Recovery was between 99.1 and 102.2 % for (1) and (2).

J. Planar Chromatogr. 37, 207-218 (2024). HPTLC of lupeol (1), stigmasterol (2) and betulin (3) in the bark and roots of Desmodium oojeinensis on silica gel with hexane - ethyl acetate 17:3. Detection by spraying with anisaldehyde - sulfuric acid reagent, followed by heating at 105 °C for 3-5 min. Quantitative determination by absorbance measurement at 520 nm. The hRF values for (1) to (3) were 41, 27 and 19, respectively. Linearity was between 200 and 600 ng/zone for (1) to (3). The inter-day and intra-day precision was below 2 % (n=6). The LOD and LOQ were 13 and 39 ng/zone for (1), 18 and 55 ng/zone for (2) and 13 and 41 ng/zone for (3), respectively. Average recovery was 97.4 % for (1), 97.7 % for (2) and 97.0 % for (3).