Cumulative CAMAG
Bibliography Service CCBS
Welcome to the Cumulative CAMAG Bibliography Service (CCBS) – your comprehensive resource for HPTLC literature. CCBS offers an up-to-date, searchable database of publications, technical papers, and application notes, curated to support research and innovation in High-Performance Thin-Layer Chromatography.
abstracts of publications 1983 -> today
Planta Medica 85(09/10), 774-780 (2019).
Summary: Preparative TLC silica gel layers were used for the purification of 8 trichothecene mycotoxins (sesquiterpenes) from 7 different subfractions obtained through column chromatography of Myrothecium roridum (Stachybotryaceae) biomass extracts.
These compounds were: (A) from hexane extract, epoxymyrotoxin A (eluent was n-hexane - ethyl acetate, 1:1); (B) from methanol extract, myrotoxin B and myrotoxin D hydrate (both from the same subfraction), as well as epoxymyrothecine A (mobile phase was dichloromethane - methanol, 9:1); (C) from ethyl acetate extract, hydroxymytoxin B, miotoxin A, roridin L-2 (eluent was dichloromethane - methanol, in 9:1; 49:1; 93:7, respectively), as well as roridin L-2 and trichoverritone (both lacking a macrocyclic lactone, isolated from each other with dichloromethane - ethyl acetate, 2:3).
J. Planar Chromatogr. 36, 433-438 (2023). HPTLC of synthetic cannabinoids AM2201 (1), CP-47,497 (2), and JWH-018 (3) in seized samples on silica gel with cyclohexane - diethylamine 9:1. Quantitative determination by absorbance measurement at 280 nm for (2) and 315 nm for (1) and (3). The hRF values for (1) to (3) were 26, 73 and 55, respectively. Linearity was between 2 and 14 µg/zone for (3), 5 and 35 µg/zone for (1) and 50 and 350 µg/zone for (2). The intermediate precision was 23.0 % for (1), 16.6 % for (2) and 7.5 % for (3). The LOD and LOQ were 5.6 and 16.9 µg/zone for (1), 0.7 and 2.2 µg/zone for (2) and 1.9 and 5.6 µg/zone for (3). Average recovery was 84.0 % for (1), 105.1 % for (2) and 107.1 % for (3).
Planta Medica 85(11/12), 917-924 (2019).
Summary: Samples were 36 methanolic extracts of Ophiopogon japonicus roots (Asparagaceae), from different ages (1-3 years) and from several locations (Sichuan and Zhejiang provinces, China). A quality control sample (QC) was made by mixing the 36 extract samples and used on each layer as reference for relative quantification. Moreover, 5 spirostanol saponins were used as standards: prosapogenin A, ophiojaponin C, ophiopogonins A, D and D' (respective hRF values were 47; 32; 50; 30 and 36).
Development on HPTLC silica gel layers with ethyl acetate - formic acid - acetic acid - water (100:11:11:27). Visualization under UV 366nm before and after derivatization by spraying with p-anisaldehyde - sulphuric acid reagent and heating 3min at 105°C. All spots of the chromatograms were quantified by normalization to QC, using an image processing software (rTLC) for data matrix extraction from the chromatogram pictures as well as for principal component analysis and OPLS-DA (orthogonal partial least squares - discriminant analysis). This allowed: A) separation of samples into 2 clusters according to the province of origin; B) separation of samples according to age. The spot of ophiopogonin D was: A) more intensive in Sichuan samples, whereas the other bands were either less strong or absent, compared to Zhejian samples; B) more intensive in younger roots.
These results were found to be similar to another multivariate data analysis performed on NMR analysis of the extracts (without TLC).
Planta Medica 85(11/12), 1024-1033 (2019).
Summary: TLC monitoring of subfractionation of the ethyl acetate (1) and butanolic (2) fractions of an ethanolic extract of aerial parts (leaves, twings, and flowers) of Halimium halimifolium (Cistaceae), through column chromatography for (1) and solid phase extraction for (2).
Development on TLC silica gel layers with chloroform - methanol (4:1) for (1), or with n-butanol - acetic acid - water (12:3:5) for (2). Derivatization with cerium sulphate - sulphuric acid reagent. The subfractions of interest contained several phenols, phenol heterosides, and flavonosides.
Chinese J. of Guangdong Chem. Ind. 50 (19), 152-155, 89 (2023)
Rehmannia glutinosa is a Chinese medicinal material, containing stachyose, manninotriose, raffinose and other oligosugar components, with pharmacological activities of regulating gastrointestinal balance, improving body immunity and endocrine, and is widely used as a core ingredient of related TCM prescriptions. In order to implement the quality control of Rehmannia glutinosa and the drugs containing Chinese medicine preparation, a TLC method for the identification of Rehmannia glutinosa was established based on the investigation and optimization of the preparation procedure of the test sample solution, chromatographic separation conditions e.g. the stationary phase and mobile phase, as well as the chromogenic means and identification of the resulting chromatograms. Under the optimized condition TLC separation of the extracts of Rehmannia glutinosa sampls to be tested, the control Rehmannia glutinosa standard sample, Rehmannia glutinosa decoction pieces samples, and the standard stachyose, manninotriose and raffinose samples on silica gel plates developed with ethyl acetate - ethanol - water - ammonia water 2 : 5 : 4 : 0.5, detection by spraying with 10% H2SO4 in ethanol and heating at 105 ºC till the chromatographic zones visualized clearly and viewing in daylight, identification by fingerprints comparison with the control Rehmannia glutinosa standard sample, Rehmannia glutinosa decoction pieces samples, and the standard stachyose, manninotriose and raffinose samples under the same condition in parallel. Applicaion to 30 batches of Rehmannia glutinosa samples and 20 batches of Rehmannia glutinosa decoction pieces samples from their origins, medicinal materials market and pharmaceutical enterprises proved the method to be simple, fast, with strong specificity, high sensitivity, throughput and robustness, thus well suitable for the title purpose.