Publications on HPTLC

A unified HPTLC screening method was developed for the analysis of twelve UV photoinitiators and benzophenone derivatives. Using a two-step development, the method provides improved differentiation across compounds with diverse chromatographic behavior and enables rapid multi-analyte screening, as demonstrated with a commercial nail polish sample.

This study, conducted by researchers at Université Clermont Auvergne (ICCF, CNRS, France), presents a new HPTLC-EDA method using a 4-methylumbelliferone (4-MU) fluorogenic probe for sensitive screening of acetylcholinesterase (AChE) inhibitors. The method provides over threefold higher sensitivity, fewer artefacts, and improved detection of bioactive compounds in complex natural extracts, offering a simple and reliable tool for bioactivity screening.

Researchers from the German Federal Institute of Hydrology and collaborating laboratories developed and validated an HPTLC-p-YES bioassay for the sensitive detection of estrogenic compounds in surface water. The method enables effect-based screening at pg/L levels, supporting comprehensive monitoring strategies in line with future European water quality standards.

This article presents a universal HPTLC method for the simultaneous separation of fourteen antimalarial active pharmaceutical ingredients and three related impurities on a single plate. The method is suitable as a rapid screening tool for pharmaceutical quality control, especially in resource-limited settings.

At Anchrom Enterprises Pvt. Ltd. in Mumbai, Mr. Parth Trivedi applies HPTLC to detect the illegal dye Rhodamine B in sweets and cosmetics. The study highlights the technique’s reliability in identifying non-permitted substances, ensuring product safety, and supporting regulatory enforcement. HPTLC proves once again to be a fast, cost-effective tool for consumer protection.

Researchers from Sazerac Switzerland GmbH and CAMAG demonstrate how HPTLC can reveal the complex composition of spirits such as whisky, rum, gin, and tequila. Using complementary developing solvents and targeted glycerol detection, the method provides rapid screening for authenticity, aging markers, and additives – helping ensure quality control and regulatory compliance in the beverage industry.

A collaborative study by Drs. Flávio L. Beltrame, Wilmer H. Perera, and Evelyn A. de Andrade highlights the use of HPTLC fingerprinting to authenticate morphologically similar Kalanchoe species. With the automated HPTLC PRO System species-specific profiles are obtained and antioxidants such as quercetin and kaempferol derivatives can be identified, offering a robust tool for quality control and standardization of medicinal plants.

Dr. Vicente L. Cebolla and colleagues at the ICB-CSIC at the University of Zaragoza use HPTLC-MS to investigate lipid composition during pennycress seed maturation. Their lipidomic profiling reveals key insights into triacylglycerol biosynthesis relevant to next-generation biofuels. The study showcases HPTLC’s unique role in combining rapid separation with detailed molecular characterization.

This optimized HPTLC method enables sensitive and reliable detection of diethylene glycol and ethylene glycol impurities in oral liquid formulations. With a detection limit as low as 21 ng and clear differentiation from other excipients, the method meets and exceeds regulatory safety thresholds, offering a robust tool for pharmaceutical quality control.

A newly optimized HPTLC method enables simultaneous resolution of 13 flavonoids and anthraquinones from leaves and flowers of seven Cassia species. Offering high specificity, fluorescence detection, and robust derivatization, this approach supports taxonomic differentiation and quality control in herbal drug analysis.