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Publications on HPTLC

Read case studies, educational materials, articles from our customer magazine CBS, and other scientific publications.

Latest from CBS Articles

Sensitive detection of estrogenic compounds in surface water

CBS Edition: 136

April 16, 2026

Researchers from the German Federal Institute of Hydrology and collaborating laboratories developed and validated an HPTLC-p-YES bioassay for the sensitive detection of estrogenic compounds in surface water. The method enables effect-based screening at pg/L levels, supporting comprehensive monitoring strategies in line with future European water quality standards.

Alan Bergmann, Schweizerisches Zentrum für angewandte Ökotoxikologie; Melanie Broszat, Hochschule Offenburg; Sebastian Buchinger, Bundesanstalt für Gewässerkunde; Marcus Flörs, Zweckverband Landeswasserversorgung Wasserwerk Langenau; Maricel Marin-Kuan, Société des Produits Nestlé; Marina Ohlig, Bundesanstalt für Gewässerkunde; Bernd Spangenberg, Hochschule Offenburg; Christel Weins, Büro für wirkungsbezogene Analytik in Umwelt und Lebensmitteln; Rebekka Weiß, Bayerisches Landesamt für Umwelt

February 17, 2026

A sensitive HPTLC-EDA method using a 4-MU fluorescent probe for AChE inhibitor screening

This study, conducted by researchers at Université Clermont Auvergne (ICCF, CNRS, France), presents a new HPTLC-EDA method using a 4-methylumbelliferone (4-MU) fluorogenic probe for sensitive screening of acetylcholinesterase (AChE) inhibitors. The method provides over threefold higher sensitivity, fewer artefacts, and improved detection of bioactive compounds in complex natural extracts, offering a simple and reliable tool for bioactivity screening.

Sensitive detection of estrogenic compounds in surface water

Researchers from the German Federal Institute of Hydrology and collaborating laboratories developed and validated an HPTLC-p-YES bioassay for the sensitive detection of estrogenic compounds in surface water. The method enables effect-based screening at pg/L levels, supporting comprehensive monitoring strategies in line with future European water quality standards.

Simultaneous separation of fourteen antimalarial active pharmaceutical ingredients and three related impurities by HPTLC

This article presents a universal HPTLC method for the simultaneous separation of fourteen antimalarial active pharmaceutical ingredients and three related impurities on a single plate. The method is suitable as a rapid screening tool for pharmaceutical quality control, especially in resource-limited settings.

November 19, 2025

Identification of Rhodamine B in food and cosmetic products

At Anchrom Enterprises Pvt. Ltd. in Mumbai, Mr. Parth Trivedi applies HPTLC to detect the illegal dye Rhodamine B in sweets and cosmetics. The study highlights the technique’s reliability in identifying non-permitted substances, ensuring product safety, and supporting regulatory enforcement. HPTLC proves once again to be a fast, cost-effective tool for consumer protection.

November 19, 2025

Analysis of spirits using HPTLC: general screening and detection of glycerol addition

Researchers from Sazerac Switzerland GmbH and CAMAG demonstrate how HPTLC can reveal the complex composition of spirits such as whisky, rum, gin, and tequila. Using complementary developing solvents and targeted glycerol detection, the method provides rapid screening for authenticity, aging markers, and additives – helping ensure quality control and regulatory compliance in the beverage industry.

November 19, 2025

HPTLC fingerprinting for distinguishing Kalanchoe species and identifying active secondary metabolites

A collaborative study by Drs. Flávio L. Beltrame, Wilmer H. Perera, and Evelyn A. de Andrade highlights the use of HPTLC fingerprinting to authenticate morphologically similar Kalanchoe species. With the automated HPTLC PRO System species-specific profiles are obtained and antioxidants such as quercetin and kaempferol derivatives can be identified, offering a robust tool for quality control and standardization of medicinal plants.

November 19, 2025

Lipidomics of Thlaspi arvense seed maturation for biofuel production

Dr. Vicente L. Cebolla and colleagues at the ICB-CSIC at the University of Zaragoza use HPTLC-MS to investigate lipid composition during pennycress seed maturation. Their lipidomic profiling reveals key insights into triacylglycerol biosynthesis relevant to next-generation biofuels. The study showcases HPTLC’s unique role in combining rapid separation with detailed molecular characterization.

Detection and limit test of diethylene glycol and ethylene glycol impurities in syrup by HPTLC

This optimized HPTLC method enables sensitive and reliable detection of diethylene glycol and ethylene glycol impurities in oral liquid formulations. With a detection limit as low as 21 ng and clear differentiation from other excipients, the method meets and exceeds regulatory safety thresholds, offering a robust tool for pharmaceutical quality control.

Simultaneous determination of flavonoids and anthraquinones in seven Cassia species

A newly optimized HPTLC method enables simultaneous resolution of 13 flavonoids and anthraquinones from leaves and flowers of seven Cassia species. Offering high specificity, fluorescence detection, and robust derivatization, this approach supports taxonomic differentiation and quality control in herbal drug analysis.

Machine learning for botanical identification

A cutting-edge machine learning system analyzes HPTLC fingerprints to accurately distinguish botanical species and common adulterants. By generating synthetic training data and automating image classification, it eliminates subjective manual comparisons – delivering faster, more reliable results for quality control in herbal products.

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