Case Study: Quantitative determination of steviol glycosides
The adage “A picture is worth a thousand words” ideally refers to the image-giving planar chromatographic method for the evaluation of samples. In this case study, an HPTLC method for analysis of steviol glycosides with its single steps was visualized in a short video recorded at the JLU Giessen, Germany. Only sugar-containing analytes, and so the steviol glycosides, were detected in separated raw extracts, Stevia formulations, or sugar-free food products using a selective derivatization with the ß-naphthol reagent. Coupling to mass spectrometry was employed for confirmation of the identity. The workflow of the method can be conveyed more effective as a visualized experiment than as a pure description. Enjoy the video and agree with the updated adage “A video is worth a thousand words”.
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