Welcome to the Cumulative CAMAG Bibliography Service (CCBS) – your comprehensive resource for HPTLC literature. CCBS offers an up-to-date, searchable database of publications, technical papers, and application notes, curated to support research and innovation in High-Performance Thin-Layer Chromatography.
12 851+
abstracts of publications 1983 -> today
12 851 abstracts found.
A new nodule-associated bacterium, Cupriavidus consociatus sp. nov., isolated from the root nodules of Leucaena sp. and Arachis sp. growing in a cacao field in Chiapas, Mexico
E.-Y. TAPIA-GARCÍA, B. CHÁVEZ-RAMÍREZ, V. LARIOS-SERRATO, I. ARROYO-HERRERA, J. ANTONIO-IBARRA, Paulina ESTRADA-de los SANTOS* (Microbiology Department, National School of Biological Sciences, National Polytechnic Institute, Mexico City, Mexico; * pestradadelossantos@gmail.com)
PLoS One 20(5), e0324390 (2025).
Samples were polar lipids extracted from Cupriavidus consociatus and C. oxalaticus (Burkholderiaceae) through the Bligh and Dyer’s technique. Two-dimensional TLC on silica gel with chloroform – methanol – water 14:6:1, followed by chloroform – methanol – acetic acid 13:5:2. Derivatization by spraying with ninhydrine for amino-lipids (phosphatidylethanolamine was detected in all strains), or with periodate Schiff’s reagent for glycolipids (phosphatidylglycerol was detected in C. consociatus, and cardiolipin was detected in both species).
Unveiling lipid and oxylipin composition in the coffee leaves throughout coffee cherry maturation
Maria FIGUEROA*, D. GUZMAN, L. NORMAND, C. VIGOR, T. DURAND, C. OGER, J. GALANO, V. GROS, G. REVERSAT, J. MALDONADO, O. GONZALEZ, M. SUAREZ, E. DURAND (*Qualisud, Univ Montpellier, Avignon Université, CIRAD, Institut Agro, Université de la Reunion, Montpellier, France, maria.figueroa@supagro.fr)
Food Chem. 493, 145746 (2025). HPTLC of lipids (free fatty acids, acylglycerols, sterols, sterol esters, glycolipids, phospholipids, tocopherol), diterpenes (cafestol and kahweol), and alkaloids (caffeine and theobromine) in coffee leaves on silica gel with chloroform - methanol - water 34:8:1 for polar compounds; chloroform - methanol - water 180:20:3 for alkaloids; and hexane - diethyl ether - acetic acid 75:25:1 for non-polar compounds. Detection by spraying with cupper sulfate - phosphoric acid - methanol - water 10:8:5:78 or thymol or ninhydrin reagent. Qualitative identification under UV light at 254 and 366 nm.
5-(3,5-Dinitrophenyl)-1,3,4-oxadiazol-2-amine derivatives, their precursors, and analogues: Synthesis and evaluation of novel highly potent antitubercular agent
V. PFLÉGR, J. STOLAŘÍKOVÁ, G. KARABANOVICH, J. MAIXNEROVÁ, A. PÁL, J. KORDULÁKOVÁ, Z. ŠANDEROVÁ, M. LIEGERTOVÁ, J. ROH, F. TREJTNAR, J. VINŠOVÁ, M. KRÁTKÝ* (Department of Organic and Bioorganic Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Hradec Králové, Czech Republic; * martin.kratky@faf.cuni.cz)
PLoS One 20(5), e0324608 (2025).
TLC on silica gel 1) to monitor the synthesis of dinitrobenzoic acid derivatives, and 2) to investigate their antimycobacterial mechanism. For 1) method A) with dichloromethane – methanol 93:7 for methyl 3,5-dinitrobenzoate (hRF 70), its hydrazide (hRF 30), and its semicarbazides = hydrazine-carboxamides (hRF 40-56) and thiosemicarbazide = hydrazine-carbothioamide (hRF 36); B) with dichloromethane – methanol 97:3 for oxadiazolamide derivatives (hRF 29-70) and for thiadiazolamide derivative (hRF 84); C) with pure chloroform or with n-hexane – ethyl acetate 1:1 for oxadiazolamines and thiadiazolamines (hRF 17-79). Detection under UV 254 nm. For 2) lipids were isolated from Mycobacterium tuberculosis (Mycobacteriaceae) after 24h culture with radioactive 14C-acetate, in a growth medium untreated or treated (four of the synthetized derivatives were tested). TLC separation with chloroform – methanol – water 40:8:1. Visualization through autoradiography in an automated imager. In treated cells, trehalose monomycolates and dimycolates were accumulated, whereas phosphatidylethanolamine (PEA) was slightly increased and cardiolipin was unchanged. Therefore, the proposed mechanism was an inhibition of epimerase DprE1.
Advanced thin‑layer chromatography‒mass spectrometry validation and comprehensive analysis of bioactive phytochemicals in Caesalpinia bonduc leaf extract
J. TANDEL, U. CHHALOTIYA*, H. KACHHIYA, D. TANDEL (*Indukaka Ipcowala College of Pharmacy, The Charutar Vidya Mandal University, Anand, India, usmangani.chhalotiya@cvmu.edu.in)
J. Planar Chromatogr. 38, 83-93 (2025). HPTLC of β-caesalpin (1) and α-caesalpin (2) in Caesalpinia bonduc leaf extract on silica gel with n-hexane - ethyl acetate 3:2. Detection by spraying with anisaldehyde reagent. Densitometry analysis at 254 and 366 nm before derivatization and at 580 nm after derivatization. Further analysis by mass spectrometry using a TLC-MS Interface. The hRF values for (1) and (2) were 64 and 78, respectively. Linearity was between 200 and 1200 ng/zone for (1) and (2). The intermediate precision was below 2 % (n=3). The LOD and LOQ were 43 and 131 ng/zone for (1) and 78 and 23 ng/zone for (2), respectively. Recovery was between 95.1 and 99.7 % for (1) and 97.5 and 99.9 % for (2).
Antimicrobial activity of soyabean (Glycine max) and pumpkin seeds (Cucurbita pepo) extracts against common oral pathogens: an in vitro study
R. NAGARAJAPPA, U. DHAR*, SATYARUP, M. JENA, A. MOHAPATRA (*Department of Public Health Dentistry, The Oxford Dental College, Bengaluru, Karnataka, India, drupasanadhar@gmail.com)
Pesqui. Bras. Odontopediatria Clin. Integr. 25, e-23221 (2025). TLC of soyabean (Glycine max) and pumpkin seeds (Cucurbita pepo) methanolic extract on silica gel with toluene - acetone - formic acid 9:9:2. Detection by spraying with aluminium chloride.