Cumulative CAMAG
Bibliography Service CCBS

J. AOAC Int. https://doi.org/10.1093/jaoacint/qsaf007 (2025). HPTLC of psilocybin (1) and psilocin (2) in Psilocybe mushroom cultivars on silica gel with 1-butanol - acetic acid - water 6:1:1. Detection by spraying with anisaldehyde reagent (20 mL of acetic acid with 170 mL of ice-cold methanol, followed by 10 mL of sulfuric acid, and finally 1 mL of anisaldehyde). Quantitative determination by absorbance measurement at 243 nm. The hRF values for (1) and (2) were 15 and 45, respectively. Linearity was 0.5 and 2.5 µg/zone for (1) and 125 and 625 ng/zone for (2). The LOD and LOQ were 64 and 193 ng/zone for (1) and 27 and 80 ng/zone for (2), respectively.

Food Chem. 489, 144682362 (2025). HPTLC of cholesteryl oleate, oleic acid methyl ester, tri-olein, oleic acid, and cholesterol in chia seeds (Salvia hispanica) on silica gel with hexane - diethyl ether - acetic acid 40:10:1. Detection by dipping into a solution of 5 % phosphomolybdic acid in ethanol, followed by heating at 100 °C for 30 min. 

J. Liq. Chromatogr. Relat. Technol. https://doi.org/10.1080/10826076.2025.2510343 (2025). HPTLC of rosmarinic acid in Rosmarinus officinalis on silica gel with toluene - ethyl acetate 4:1. Quantitative determination by absorbance measurement at 366 nm. The hRF value for rosmarinic acid was 55. Linearity was between 4 and 12 µg/zone. The intermediate precision was below 2 % (n=3). The LOD and LOQ were 289 and 965 ng/zone, respectively. Average recovery was 89.7 %.

J. AOAC Int. https://doi.org/10.1093/jaoacint/qsaf063 (2025). HPTLC of cranberry supplements on silica gel with ethyl acetate - water - formic acid - glacial acetic acid 100:27:11:11. Detection by dipping into Natural Products Reagent, followed by heating at 100 °C for 3 min. Qualitative analysis under UV light at 366 nm. Digitized chromatograms were exported into Excel and processed in MATLAB R2021a and Microsoft Excel. Preprocessing steps included normalization, retention factor (hRF) alignment, and data integration across derivatization conditions. Digitization of HPTLC chromatograms, followed by hRF alignment and normalization, effectively addressed the variability of HPTLC analysis.

Phytochem. Anal. https://doi.org/10.1002/pca.70000 (2025). Review of recent developments and the evolution of modern quality control techniques, such as two dimensional assays, UHPLC, spectroscopy, mass spectrometry, and genomics analyses to avoid toxic or allergenic effects from herbal materials that are used as components of dietary supplement products. The paper described TLC and HPTLC methods in combination with mass spectrometry for the analysis of secondary metabolites  for regulatory compliance, particularly for herbal raw materials.